This assay is used, as a follow up testing to an abnormal hemoglobin HPLC/ electrophoresis, for elucidating ? thalassemia genotypes. Amplified DNA from the ? thalassemia patient samples are probed for mutations using a line probe assay. Genetic abnormalities specific for ? thalassemia like 3.7 single gene deletion, 2 IVS1 (-5nt) mutation, etc can be detected by using an Amplification refractory mutation system (ARMS) PCR.