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17 Hydroxy Progesterone

Test Code: 478 CPT Code: 83498
Specimen Type: Serum (yellow-top tube)
Sample Volume: 3.0 mL (1.0mL - min)
Transport Condition: Refrigerated/ frozen condition
Specimen Rejection Criteria: Gross hemolysis
Methodology: Immunoassay
Special Requirements: Age & Gender for the patient is mandatory
Clinical Significance:

Serum 17-alpha-OHP is characteristically elevated in those cases of congenital adrenal hyperplasia which are due to
21or 11-hydroxylase deficiency. The block in the glucocorticoid pathway results in an accumulation of 17-alpha-OHP and an inhibition of cortisol formation. Adrenocorticotrophin (ACTH) production increases in response to the cortisol deficiency, which in turn, enhances the formation of adrenal androgens.

Run Day: Mon, Thurs
Reporting Day: Same day

25-OH Vitamin D3 & D2, HPLC analysis

Test Code: 6930 CPT Code: 82306
Specimen Type: Serum collected in a yellow-top tube
Sample Volume: 3.0 mL (1.0mL - min)
Transport Condition: Room temperature
Specimen Rejection Criteria: Gross hemolysis, gross lipemia
Methodology: HPLC
Special Requirements: No special precautions needed
Clinical Significance:

This assay should be used to monitor patients taking vitamin D supplements as well as to diagnose vitamin D deficiency. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-OHD: 25-OHD3 the endogenous form of the vitamin; and 25-OHD2 the analog form used to treat 25-OHD3 deficiency.

Run Day: Daily
Reporting Day: Same day

5Hydroxyindoleacetic Acid(5-HIAA), 24 Hour, Urine

Test Code: 13210 CPT Code: 83497
Specimen Type: 24 hrs urine
Sample Volume: 20 mL
Transport Condition: Frozen Strictly
Specimen Rejection Criteria: Non frozen specimen. Not protected from light. Improper preservative
Methodology: Enzyme immunoassay
Special Requirements: Following instructions must be strictly followed: 24 hrs urine to be collected in 15 mL of 6N HCL. While collecting specimen, the container should be kept in dark throughout the collection. To avoid exposure to light, the 20 mL aliquot from the collected 24 hrs urine sample must be wrapped in aluminum foil. Do not consume bananas, pineapple, and nuts 48 hrs prior to the collection of the specimen. Mention clinical details (CT scan, USG & medication) of the patient on the request form.
Clinical Significance:

5-Hydroxyindoleacetic acid (5HIAA) is the major metabolite of serotonin and is excreted in the urine. Intestinal carcinoid tumors and neuroendocrine tumors
can produce high amounts of 5-HIAA and serotonin, especially in patients with carcinoid syndrome. Assay of 5-HIAA in a 24-hour urine specimen can aid in diagnosis of carcinoid disease
with a high specificity.

Run Day: Tue
Reporting Day: Next day